ANATOMICAL VARIATIONS OF THE PARIETAL FORAMEN AND ITS RELATIONS TO THE CALVARIAL LANDMARKS: A CROSS-SECTIONAL CADAVERIC STUDY.

OBJECTIVE: The aim: Estimate the prevalence of the parietal foramen in the adult human skulls of Ukrainian origin, and study its morphology and relationships to main anatomical landmarks of the skull. PATIENTS AND METHODS: Materials and methods: A cross-sectional observational study of PF was conducted with 42 random cadaveric adult human skull roofs (calvaria) collected from the laboratory and museum of Human Anatomy Department, Kharkiv National Medical University, Ukraine. The patency and the length of the PF canal were determined, and PF external/internal diameters and the distance to the calvarial landmarks from PF were measured using the caliper. Mean and standard deviation were calculated to compare with the existing data. RESULTS: Results: In the present study 85.7% (n = 36) of the calvaria had the PF, 54.8% (n = 23) had bilateral location of PF, 30.9% (n = 13) had unilateral presence of PF (right side: 23.8%, n=10 and left side: 7.1%, n=3), and 14.3 % (n = 6) demonstrated bilateral absence of PF. CONCLUSION: Conclusions: An anatomical variation in parietal foramen is not uncommon, and the differences can be based on multiple factors like geography and race. It is important to have detailed information on anatomical variations in different population groups to facilitate surgical and radiological interventions.

STUDY OF ANTIBACTERIAL ACTIVITY OF THE PREPARATIONS WITH DIOXIDINE AND LEVOFLOXACIN ON MAIN PURULENT-INFLAMMATORY PROCESSES PATHOGENS.

OBJECTIVE: The aim: Study of antibacterial activity of the preparations, containing antiseptic dioxidine and antibiotic levofloxacin in vitro on standard strains of main optional-anaerobic pathogens of purulent-inflammatory processes of surgical wounds S. aureus, E. coli, P. aeruginosa and definition of more effective ones on them. PATIENTS AND METHODS: Materials and methods: Solutions of dioxidine 1.2 %, dioxidine 1.2% with decamethaxin, Dioxisole, water soluble ointment with dioxidine 1.2% and levofloxacin 0.1% with decamethaxin were used in experiment. Antibacterial activity was studied on standard strains of S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Distinguishing and identification of pure cultures of bacteria was done according to generally accepted microbiological methods. Determination of purulent-inflammatory processes pathogens sensitivity was done by disco-diffuse method on Mueller-Hinton medium. Antibacterial activity of solutions and ointments was studied with the help of agar diffusion method ("well" method) according to methodic recommendations. Each investigation was repeated 6 times. Method of variation statistics was used for the research results analysis. RESULTS: Results: All antibacterial preparations under study are effective and highly effective on S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin have larger growth retardation zones towards S. aureus and P. aeruginosa. E. coli strains are more sensitive to the solution of Dioxisole and ointment with 1.2 % dioxidine. CONCLUSION: Conclusions: All strains are sensitive, most of them are highly sensitive, up to 5 antibacterial preparations under study in vitro.

Study of pathogenic factors of e.coli isolated from patients with peritonitis.

OBJECTIVE: The aim: To study the biological properties of museum and clinical strains of E.coli isolated from patients with peritonitis. PATIENTS AND METHODS: Materials and methods: It was used 94 strains (clinical, museum and reference). The ability of E. coli to adhere was investigated by hemadhezive method to formal human erythrocytes of 0 (I) Rh-positive blood group. The study measured the ability of microorganisms to produce gelatinaze, caseinase, fibrinolysin, hemolysin. To control of the enzyme activity the positive and negative control with reference strains were used. Synchronisation of cultures activity before experiments was achieved by one-time effect of low temperature (+4 С) during 30 minutes. RESULTS: Results: To investigate the pathogenic factors of E. coli we carried out determining of proteolytic, gelatinous, caseinous, fibrinolytic, haemolytic and adhesion properties. CONCLUSION: Conclusions: In our investigation pathogen icspecies of Esherihies are virtually indistinguishable from representatives of normal microflora on its morphological, biochemical and cultural properties. During investigation of serological properties of selected of E. coli strains (n = 94) 65.8% of pathogenic serotypes were revealed. Moreover, all marked E. coli isolated from the abdominal cavity of children and adults, as well as museum strains related to enteropathogenic E. coli (O127: K63, O33: K-) and 1 - to enteroinvazive Escherichia coli (O144: K-).